Journal: Redox Report : Communications in Free Radical Research

Article Title: Renal mitochondrial oxidative stress is enhanced by the reduction of Sirt3 activity, in Zucker diabetic fatty rats

doi: 10.1080/13510002.2018.1487174

Figure Lengend Snippet: Characteristics of rats and changes in renal fibrosis and tubular cell damage at 28 weeks of age. (A) body weight, (B) levels of HbA1c, (C) kidney weight, (D) urinary albumin/creatinine (Cr) ratio, (E) liver-type free fatty acid-binding protein (L-FABP)/Cr and (F) 8-hydroxy-2'-deoxyguanosine (8-OHdG)/Cr ( n = 6, respectively). (G) Representative photographs of Masson’s Trichrome (MT) staining (scale bar: 100 μm), immunohistochemistry for kidney injury molecule-1 (Kim-1) (scale bar: 100 μm). Quantitative data of fibrosis area (H) on MT staining and Kim-1-positive area (I) on Kim-1 immunohistochemistry per visual field of tubulo-interstitial area (MT: n = 6, Kim-1 IHC: n = 4). mRNA expression of type 3 collagen (Collagen III) (J) and Kim-1 (K), adjusted to the expression level of 18s in the renal cortex ( n = 6, respectively). All data are mean ± SD. # p < .01, ## p < .05, n.s. denotes not significant. ZDFRs: Zucker diabetic fatty rats, ZLRs: Zucker lean rats.

Article Snippet: Male Zucker Lean (fa/+) Rats (ZLRs) and male ZDFRs were provided by the Ninox Pharmaceutical Company Biological Institute (Osaka, Japan).

Techniques: Binding Assay, Staining, Immunohistochemistry, Expressing

Journal: Redox Report : Communications in Free Radical Research

Article Title: Renal mitochondrial oxidative stress is enhanced by the reduction of Sirt3 activity, in Zucker diabetic fatty rats

doi: 10.1080/13510002.2018.1487174

Figure Lengend Snippet: Change in Sirt3 activity, oxidative stress in mitochondria and mitochondrial morphology in the type 2 diabetic kidney. (A) Western blots of mitochondrial protein extracts from ZLRs and ZDFRs with polyclonal anti-lysine acetylation or Cytochrome C Oxidase Subunit IV (CoxIV) antibodies. (B) Representative western blots of acetylated isocitrate dehydrogenase 2 on Lys-413 (Ace-IDH2), IDH2, acetylated superoxide dismutase 2 on Lys-68 (Ace-SOD2), SOD2, Sirt3, dynamin-related protein 1 (DRP1) and CoxIV in mitochondrial protein extracts from ZLRs and ZDFRs. Quantitative ratios of Ace-IDH2 to IDH2 (C), Ace-SOD2 to SOD2 (D), Sirt3 (E) and DRP1 (F) to CoxIV ( n = 6, respectively). (G) 8-OHdG content of mitochondrial DNA from ZLRs and ZDFRs ( n = 6). (H) Representative transmission electron microscopy images of proximal tubular cells (scale bar: 500 nm). All data are mean ± SD. # p < .01, ## p < .05, n.s. denotes not significant. ZDFRs: Zucker diabetic fatty rats, ZLRs: Zucker lean rats.

Article Snippet: Male Zucker Lean (fa/+) Rats (ZLRs) and male ZDFRs were provided by the Ninox Pharmaceutical Company Biological Institute (Osaka, Japan).

Techniques: Activity Assay, Western Blot, Transmission Assay, Electron Microscopy

Journal: Redox Report : Communications in Free Radical Research

Article Title: Renal mitochondrial oxidative stress is enhanced by the reduction of Sirt3 activity, in Zucker diabetic fatty rats

doi: 10.1080/13510002.2018.1487174

Figure Lengend Snippet: Change in renal expression of CD38 and NAD + /NADH ratio in type 2 diabetic rats. (A) Representative photographs of immunohistochemistry for CD38 in the tubule-interstitial area (scale bar: 200 μm) and glomeruli (scale bar: 100 μm). Quantitative data of CD38-positive area per visual field in tubulo-interstitial area (B) and in glomeruli (C) on IHC ( n = 4, respectively). (D) Representative western blots of CD38 and β-actin in renal cortex protein extracts from ZLRs and ZDFRs. (E) Quantitative ratios of CD38 to β-actin ( n = 6). (F) mRNA expression of CD38, adjusted to the expression level of 18s in the renal cortex ( n = 6). (G) NAD + /NADH ratio in the renal cortex of ZLRs and ZDFRs ( n = 6). (H) Representative western blots of CD38 and CoxIV in mitochondria protein extracts isolated from renal cortex of ZLRs and ZDFRs. (I) Quantitative ratios of CD38 to CoxIV ( n = 6). All data are mean ± SD. ## p < .05. ZDFRs: Zucker diabetic fatty rats, ZLRs: Zucker lean rats.

Article Snippet: Male Zucker Lean (fa/+) Rats (ZLRs) and male ZDFRs were provided by the Ninox Pharmaceutical Company Biological Institute (Osaka, Japan).

Techniques: Expressing, Immunohistochemistry, Western Blot, Isolation

Journal: Aging (Albany NY)

Article Title: CD38 inhibition by apigenin ameliorates mitochondrial oxidative stress through restoration of the intracellular NAD + /NADH ratio and Sirt3 activity in renal tubular cells in diabetic rats

doi: 10.18632/aging.103410

Figure Lengend Snippet: Apigenin ameliorates renal fibrosis and inflammatory gene expression in diabetic rats. ( A ) Representative photographs of MT staining (scale bar: 100 μm) and IHC of Kim-1 in the tubulointerstitial area (scale bar: 100 μm) and the mitochondrial morphology observed under transmission electron microscopy (TM) scale bar: 500 nm). ( B – E ) Quantitative RT-PCR of collagen III ( B ), Kim-1 ( C ), TNF-α ( D ), and IL-6 ( E ) mRNA normalized to expression of 18S, in the renal cortex (n=6). All data represent the mean ± standard deviation (SD). *p<0.01 vs. other groups. ZLRs; Zucker lean rats, ZDFRs; Zucker diabetic fatty rats.

Article Snippet: Male Zucker Lean ( fa/+ ) Rats (ZLRs) and male Zucker Diabetic Fatty ( fa/fa ) Rats (ZDFRs) were provided by the Ninox Pharmaceutical Company Biological Institute (Osaka, Japan) [ ].

Techniques: Gene Expression, Staining, Transmission Assay, Electron Microscopy, Quantitative RT-PCR, Expressing, Standard Deviation